A new mutation affecting ribosomal RNA synthesis in Escherichia coli
β Scribed by Chaney, Stephen G.; Jackson, James M.; Harris, Jean S.
- Book ID
- 126092330
- Publisher
- American Chemical Society
- Year
- 1977
- Tongue
- English
- Weight
- 566 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0006-2960
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π SIMILAR VOLUMES
A new ribosomal mutant resistant to erythromycin is described. The product of the gene eryC seems to be implicated in the assembly of the two ribosomal subunits, particularly in the maturation process of the RNA 23S and 16S.
Ribosomal RNA synthesis has been investigated in an in vitro system consisting of purified E. coli RNA polymerase and phi 80d3 DNA carrying rrnC operon. rRNA comprises about 25% of the total RNA synthesized in this system under optimal conditions and is stimulated by a crude protein fraction prepare
Nucleoids were isolated from Escherichia coli B/r cells in steady-stage growth at different rates. The number of RNA chains growing on each nucleoid was estimated from the amount and size of RNAs synthesized by endogenous RNA polymerase. This figure represents the number of RNA polymerase molecules