A new method to obtain β-glucan from Saccharomyces cerevisiae cells
✍ Scribed by Ferreira, Tatiana Felix; de Andrade, Leonardo Rodrigues; Coelho, Maria Alice Zarur; da Rocha-Leão, Maria Helena Miguez
- Book ID
- 125516988
- Publisher
- The Royal Society of Chemistry
- Year
- 2011
- Tongue
- English
- Weight
- 816 KB
- Volume
- 1
- Category
- Article
- ISSN
- 2044-4753
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This report describes a method for the solubilization of a micro-particulate beta-D-glucan. Insoluble glucan is dissolved in methyl sulfoxide and urea (8M) and partially phosphorylated at 100 degrees. The resulting water-soluble product is called glucan phosphate. The conversion rate is 70%, and the
In yeast and other fungi, cell division, cell shape, and growth depend on the coordinated synthesis and degradation of cell wall polymers. We have developed a reliable and efficient micro method to determine Saccharomyces cerevisiae cell wall composition that distinguishes between 1,3and 1,6-gluca