A new method to measure the specific cellular component of a delayed hypersensitivity response in the ear of the mouse
β Scribed by D. Sabolovic; Marie-Chantal Beugnot; F. Dumont; Madeleine Bujadoux
- Publisher
- John Wiley and Sons
- Year
- 1972
- Tongue
- English
- Weight
- 282 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0014-2980
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β¦ Synopsis
Abstract
We here describe a simple method of measuring a pure cellular reaction in the mouse.
Mice were challenged at the ear pinna to detect a cell population labeled in vivo with [^3^ H]thymidine. The reaction was found to be specific, radiosensitive and thymusβdependent.
π SIMILAR VOLUMES
A new, rapid method for the measurement of [y3\*P]ATP specific radioactivity in tissue extracts in the presence of other 32P-containing compounds is described. The deproteinized extract is incubated with phosphorylase b and phosphorylase kinase, and the incorporation of 3zP into protein from [Y-~\*P