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A new method to measure the specific cellular component of a delayed hypersensitivity response in the ear of the mouse

✍ Scribed by D. Sabolovic; Marie-Chantal Beugnot; F. Dumont; Madeleine Bujadoux


Publisher
John Wiley and Sons
Year
1972
Tongue
English
Weight
282 KB
Volume
2
Category
Article
ISSN
0014-2980

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✦ Synopsis


Abstract

We here describe a simple method of measuring a pure cellular reaction in the mouse.

Mice were challenged at the ear pinna to detect a cell population labeled in vivo with [^3^ H]thymidine. The reaction was found to be specific, radiosensitive and thymus‐dependent.


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✍ Paul J. England; Donal A. Walsh πŸ“‚ Article πŸ“… 1976 πŸ› Elsevier Science 🌐 English βš– 438 KB

A new, rapid method for the measurement of [y3\*P]ATP specific radioactivity in tissue extracts in the presence of other 32P-containing compounds is described. The deproteinized extract is incubated with phosphorylase b and phosphorylase kinase, and the incorporation of 3zP into protein from [Y-~\*P