✦ LIBER ✦

A New Method of Bile Acid Silylation for Their GLC–MS Analysis

✍ Scribed by M. N. Bakola-Christianopoulou; K. K. Apazidou; L. Psarros

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 287 KB
Volume: 10
Category: Article
ISSN: 0268-2605
DOI: 10.1002/(sici)1099-0739(199609)10:7<531::aid-aoc511>3.0.co;2-l

No coin nor oath required. For personal study only.

✦ Synopsis

The trimethylsilyl (TMS) derivatives of a mixture of nine bile acids (six free and three conjugated), namely lithocholic, deoxycholic, chenocholic, cholic, hyodeoxycholic, ursodeoxycholic, glycodeoxycholic, glycocholic and glycochenodeoxycholic acids, have been prepared by a new, simple, efficient derivatization procedure, based on the use of a mixture of N-methyl-N-trimethylsilyl-l,l,ltrifluoroacetamide and 1-(trimethylsi1yl)imidazole, as the silylating agent. The above-mentioned bile acids were completely trimethylsilylated on all hydroxyl and carboxyl groups whereas carbonyl and amino groups remained untouched.

📜 SIMILAR VOLUMES

A new method for the analysis of styrene

A new method for the analysis of styrene mercapturic acids by liquid chromatography/electrospray tandem mass spectrometry

✍ Paola Manini; Roberta Andreoli; Enrico Bergamaschi; Giuseppe De Palma; Antonio M 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 81 KB 👁 2 views

A New High Performance Liquid Chromatogr

A New High Performance Liquid Chromatography Method for the Simultaneous Quantitative Analysis of Pyrrolizidine Alkaloids and their N-Oxides in Plant Material

✍ G. Hösch; H. Wiedenfeld; Th. Dingermann; E. Röder 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 472 KB 👁 2 views

A new high performance liquid chromatography (HPLC) method is described for the simultaneous quantitative determination of pyrrolizidine alkaloids and their N-oxides. The method is based on ion pair HPLC using LiChrospher 60 RP-select B as the stationary phase. The method has been applied to the ana