A new method is described which enables the visualization of hidden cellular surfaces of biological tissues. The method does not depend on the kind of fixative solution, the duration of the fixation, or the quantity of extracellular matrix. The samples are chemically dissociated with potassium ethox
✦ LIBER ✦
A New Method for Three-Dimensional Reconstruction of Single Macromolecules Using Low-Dose Electron Micrographs
✍ Scribed by JOACHIM FRANK; MICHAEL RADERMACHER; TERENCE WAGENKNECHT; ADRIANA VERSCHOOR
- Book ID
- 118723760
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 740 KB
- Volume
- 483
- Category
- Article
- ISSN
- 0890-6564
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new method is described for three-dimensional reconstruction from serial ultrathin sections, using "meta-balls" as a primitive of object modeling in computer graphics (CG). We take advantage of the meta-ball's blobbiness characteristic and its rendering system for reconstructing images. The two-dime