A New Method for Quantitative Real-Time Polymerase Chain Reaction Data Analysis
โ Scribed by Rao, Xiayu; Lai, Dejian; Huang, Xuelin
- Book ID
- 121295380
- Publisher
- Mary Ann Liebert
- Year
- 2013
- Tongue
- English
- Weight
- 167 KB
- Volume
- 20
- Category
- Article
- ISSN
- 1066-5277
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๐ SIMILAR VOLUMES
## Abstract An efficient and effective method for quantification of small amounts of nucleic acids contained within a sample specimen would be an important diagnostic tool for determining the content of mitochondrial DNA (mtDNA) in situations where the depletion thereof may be a contributing factor
Real-time reverse transcription (RT) PCR is currently the most sensitive method for the detection of low-abundance mRNAs. Two relative quantitative methods have been adopted: the standard curve method and the comparative C T method. The latter is used when the amplification efficiency of a reference