A new method for drug transport studies on pig nasal mucosa using a horizontal Ussing chamber
✍ Scribed by Karin Östh; Johan Gråsjö; Erik Björk
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 286 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0022-3549
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✦ Synopsis
The horizontal Ussing chamber method described here allows performance of transport studies on pig nasal respiratory mucosa under conditions simulating reality in that it mimics the air±mucosa interface. The transport of testosterone and mannitol through pig nasal mucosa in the horizontal Ussing chamber was investigated using both liquid and air mucosal interfaces. There were no signi®cant differences in either the bioelectrical parameters (transmucosal electrical resistance, R, potential difference, PD, and short circuit current, I sc ) or the apparent permeability (P app ) of the mucosa to testosterone or mannitol between the liquid and air interface experiments. The histological study showed that the epithelial cell layer tolerates exposure to the air interface well. The P app equation was developed to correct for substance binding to the wall of the receiver chamber. The mean values AE SD of R, PD, and I sc for the mucosae in the study were 75.0 AE 28.0 Ocm 2 , (À4.53) AE 3.46 mV and 58.6 AE 28.8 mA/cm 2 , respectively. The corrected P app for testosterone with and without the mucosal air interface were 9.82 Á 10 À6 AE 11.41 Á 10 À6 cm/s and 32.24 Á 10 À6 AE 31.12 Á 10 À6 cm/s, respectively. The P app values for mannitol with and without the air interface were 2.26 Á 10 À6 AE 1.42 Á 10 À6 cm/s and 3.12 Á 10 À6 AE 1.72 Á 10 À6 cm/s, respectively.