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A new method for determination of urinary citrate

✍ Scribed by Kodama, M. ;Kohri, K. ;Iguchi, M. ;Kato, Y. ;Takada, M. ;Katayama, Y. ;Ishikawa, Y. ;Umekawa, T. ;Yachiku, S. ;Kurita, T.

Publisher
Springer
Year
1992
Tongue
English
Weight
241 KB
Volume
20
Category
Article
ISSN
0300-5623

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✦ Synopsis

We have developed a new assay technique using high-performance liquid chromatography. The assay was performed at a flow rate of 0.7 ml/min, a temperature of 60 degrees C and an ultraviolet absorption of 214 nm. Comparison of the results of the new assay with the results obtained for identical samples using the conventional fluorometric method demonstrated a very high correlation coefficient of 0.931.

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A simple and rapid technique for the determination of oxalate in urine by ion chromatography has been described. Although there is difficulty in separating the oxalate peak from the sulfate peak on the conductivity chromatogram of unprocessed urine, it is possible to eliminate the sulfate peak by th