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A new method for counting labeled nucleic acids by liquid scintillation

โœ Scribed by Anthony Trewavas

Publisher
Elsevier Science
Year
1967
Tongue
English
Weight
343 KB
Volume
21
Category
Article
ISSN
0003-2697

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Complete extraction in a form suitable f
Complete extraction in a form suitable for liquid scintillation counting of tritium-labeled proteins from polyacrylamide gels
โœ Clive Meredith; Martin K. Johnson ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 344 KB

Large (200 mm3) slices of polyacrylamide gels crosslinked with N,N'-diallyltartardiamide which contain tritium-labeled protein are readily solubilized in periodic acid for liquid scintillation counting of radioactivity, but the apparent recovery of label never exceeds 82%. Extraction of the slices w