✦ LIBER ✦

A new method for assaying peptidyl-tRNA hydrolase

✍ Scribed by William V. Zucker

Publisher: Elsevier Science
Year: 1975
Tongue: English
Weight: 292 KB
Volume: 63
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(75)90376-0

No coin nor oath required. For personal study only.

✦ Synopsis

A new assay is described for the activity of peptidyl-tRNA hydrolase on naturally occurring peptidyl-tRNA molecules. It takes advantage of the differential binding of the tRNA and peptide moieties to DEAE paper.

📜 SIMILAR VOLUMES

Development of a Fluorescence Polarizati

Development of a Fluorescence Polarization Assay for Peptidyl–tRNA Hydrolase

✍ Paul D. Bonin; Laurence A. Erickson 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 121 KB

Peptidyl-tRNA hydrolase (Pth) activity ensures the rapid recycling of peptidyl-tRNAs that result from premature termination of translation. Historically, the hydrolyzing activity of Pth has been assayed with radiolabeled N-blocked aminoacyl-tRNAs in assay systems that require the separation of radio

New method for assaying antiapomorphine

New method for assaying antiapomorphine activity in pigeons

✍ W. K. Van Tyle; A. M. Burkman 📂 Article 📅 1970 🏛 John Wiley and Sons 🌐 English ⚖ 393 KB

A microdetermination method for assaying

A microdetermination method for assaying glycosaminoglycans and proteoglycans

✍ P.Mark Bartold; Roy C. Page 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 422 KB

A simple and reliable method is described for the determination of glycosaminoglycans and proteoglycans in tissue extracts as well as during preparative and analytical procedures for these molecules. It is particularly useful because it requires much less starting material for the identification of

A colorimetric method for α-naphthol and

A colorimetric method for α-naphthol and its application to assay of hydrolases

✍ Bernard Weissmann 📂 Article 📅 1969 🏛 Elsevier Science 🌐 English ⚖ 310 KB

A DEAE-cellulose filter disk assay for a

A DEAE-cellulose filter disk assay for aminoacyl-tRNA

✍ Daniel V. Santi; Robert T. Anderson Jr. 📂 Article 📅 1974 🏛 Elsevier Science 🌐 English ⚖ 459 KB

A method for assaying the rhamnosidase a

A method for assaying the rhamnosidase activity of naringinase

✍ Carmen Romero; Arturo Manjón; Josefa Bastida; JoséL. Iborra 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 469 KB

The use of the p-nitrophenyl-cu-L-rhamnopyranoside for the specific measurement of the (Yrhamnosidase activity of naringinase, by calorimetrically following the appearance of p-nitrophenolate anion, is proposed. Use of this synthetic substrate did not change the pH, temperature, or ionic strength op