A new measure of cooperativity in protein-ligand binding
β Scribed by W.E. Briggs
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 381 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0301-4622
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## Abstract Positive cooperativity, defined as an enhancement of the ligand affinity at one site as a consequence of binding the same type of ligand at another site, is a free energy coupling between binding sites. It can be present both in systems with sites having identical ligand affinities and
A double conical gel-permeation column is described, which permits an efficient dispersion of solutes in passage. It makes possible a facile determination of ligand binding to protein on the basis of the differential gel permeation of these molecules. The system has been applied, using Sephadex G-25