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A new ligand for hydrophobic charge induction chromatography

✍ Scribed by Guofeng Zhao; Fuqiang Li; Guanying Peng; Qinghong Shi; Yan Sun

Book ID
118472698
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
73 KB
Volume
136
Category
Article
ISSN
0168-1656

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## Abstract Histamine was immobilized on Sepharose CL‐6B (Sepharose) for use as a ligand of hydrophobic charge induction chromatography (HCIC) of proteins. Lysozyme adsorption onto Histamine‐Sepharose (HA‐S) was studied by adsorption equilibrium and calorimetry to uncover the thermodynamic mechanis