A new human synovial sarcoma cell line, HS-SY-3, with a truncated form of hybrid SYT/SSX1 gene

✍ Scribed by Hiroshi Sonobe; Tamotsu Takeuchi; Sheng-Ben Liag; Takahiro Taguchi; Kazunari Yuri; Kiichi Shimizu; Jun Iwata; Mutsuo Furihata; Yuji Ohtsuki; Joseph R. Testa

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: French
• Weight: 266 KB
• Volume: 82
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19990730)82:3<459::aid-ijc21>3.0.co;2-b

✦ Synopsis

Recent cytogenetical and molecular studies have indicated that synovial sarcoma harbors a t(X;18)(p11.2;q11.2) translocation, resulting in the formation of a hybrid SYT/SSX (SSX1 or SSX2) gene. We newly established a human cell line, HS-SY-3, from a synovial sarcoma. HS-SY-3 cells were shown to harbor the pathognomonic t(X;18)(p11.2;q11.2) translocation by chromosome analysis but not to exhibit the classical hybrid SYT/SSX transcripts induced by this translocation, using RT-PCR. To determine the reason for this discrepancy, we analyzed cDNA from HS-SY-3 cells, as well as the original sarcoma tissue by the rapid amplification of cDNA 3Ј end assay, and found that the chimaeric cDNA was 240 bp shorter than the previously established SYT/SSX1 cDNA due to truncation of the 3Ј side of SSX1. The HS-SY-3 cells should be useful for future functional studies of the SYT/SSX chimeric gene.