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A new highly sensitive detection system for peptides and proteins in column effluents

โœ Scribed by J.J.T. Gerding; H.J.M. Kempe; B.J.M. Lamer; M.H. Gerdin


Book ID
118532085
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
738 KB
Volume
66
Category
Article
ISSN
1873-3778

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The trinitrobenzenesulfonic acid (TNBS) method of R. Fields (1971, Biochem. J., 124, 581-590) has been modified for the manual detection of amino acids and peptides in chromatographic column effluent by changing the reaction conditions to 1 mM TNBS in 0.4 M potassium borate buffer, pH 9.2, at room