✦ LIBER ✦
A new general approach to fluorogenic enzyme assays of high sensitivity and potential high specificity, illustrated by a procedure for β-d-galactosidase estimations
✍ Scribed by Stephen Creme; David H. Leaback
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 436 KB
- Volume
- 103
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A novel fluorogenic substrate (methylumbelliferyl2-acetamido-2-deoxy-~-o-lactoside) has been prepared enzymatically. A procedure has been developed for its use as a convenient and sensitive fluorogenic substrate for /3-D-galactosidase assay with a potential for high substrate specificity. The merits of this new fluorogenic substrate for /3-D-galactosidase assays are discussed, together with the potential of this approach for a wider range of enzyme activities.