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A New Fluorescence Resonance Energy Transfer Pair and Its Application to Oligonucleotide Labeling and Fluorescence Resonance Energy Transfer Hybridization Studies

✍ Scribed by Michaela Gruber; Bianca Wetzl; Bernhard Oswald; Joerg Enderlein; Otto S. Wolfbeis


Book ID
106412231
Publisher
Springer
Year
2005
Tongue
English
Weight
148 KB
Volume
15
Category
Article
ISSN
1053-0509

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Fluorescence resonance energy transfer b
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## Abstract We use fluorescein as the energy donor and rhodamine as the acceptor to measure the efficiency of fluorescence resonance energy transfer (FRET) in a set of hybridized DNA constructs. The two fluorophores are covalently attached via linkers to two separate oligonucleotides with fluoresce