A New Fluorescence Microscopy for Tomographic Observation of Microcirculation by Using Dual-Beam Slit Laser Illumination

Use of a new fluorescent probe, seminaph

Use of a new fluorescent probe, seminaphthofluorescein-calcein, for determination of intracellular pH by simultaneous dual-emission imaging laser scanning confocal microscopy

✍ You Zhou; Elizabeth M. Marcus; Richard P. Haugland; Michal Opas 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 785 KB

A new p H indicator, seminaphthofluorescein (SNAFL)-calcein acetoxymethyl ester, was used for intracellular p H (pH,) measurement in living MDCK cells with a laser scanning confocal microscope (LSCM) equipped with an Argon/Krypton laser and dual-excitation and dual-emission (FITC/Texas Red) filter s