A new fluorescence enhancement solution for Europium-based time-resolved fluoroimmunoassays

✍ Scribed by Degan, Paolo ;Abbondandolo, Angelo ;Montagnoli, Giorgio

• Publisher: John Wiley and Sons
• Year: 1990
• Weight: 467 KB
• Volume: 5
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170050312

✦ Synopsis

A cationic detergent is proposed as suitable insulating agent for the preparation of a fluorescence enhancement solution useful for europium-based time-resolved fluoroimmunoassays. Luminescence from europium ions at concentration as low as 0.5pmol/l can be detected in solution containing 1.6 mmol/l thenoyltrifluoroacetone, 110.5 p mol/l Adogen 464 and 0.1% Tween 20, in the presence of 0.5mol/l NaCI. A competitive TR-FIA for rabbit IgG is described, showing that the new enhancement solution allows a sensitivity Comparable to that of the high performance LKB Wallac DELFIA.