A convenient lactic dehydrogenase-couple
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Stephen T. Worland; Jules A. Shafer
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Article
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1980
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Elsevier Science
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English
β 715 KB
An assay for determining the concentration of pyridoxal Y-phosphate in plasma from 0.4 ml whole blood is reported. The assay consists of incubating deproteinized plasma with o-setine apodehydratase from Escherichia cob' in 0.5 M N-2-hydroxyethylpipetazine-N'-3-propesulfonic acid, pH 7.8, at 37Β°C for