A new enzymic defect of galactose metabolism in Escherichia coli K-12 mutants
โ Scribed by T. Fukasawa; K. Jokura; K. Kurahashi
- Book ID
- 118847917
- Publisher
- Elsevier Science
- Year
- 1962
- Tongue
- English
- Weight
- 261 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0006-291X
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Two routes of one-carbon biosynthesis have been described in Escherichia coli K12. One is from serine via the serine hydroxymethyltransferase (SHMT) reaction, and the other is from glycine via the glycine cleavage (GCV) enzyme system. To isolate mutants deficient in the GCV pathway, we used a select
From a collection of 8,000 transposon-insertion mutants of Escherichia coli K12 we identified two mutations, trg-1::Tn5 and trg-2::Tn10, that simultaneously eliminate chemotactic response to ribose and galactose, two attractants recognized by independent receptors. We show that these transposon-inse