A new electrochemical assay method for gene expression using hela cells with a secreted alkaline phosphatase (SEAP) reporter system

High cell density fed batch and perfusio

High cell density fed batch and perfusion processes for stable non-viral expression of secreted alkaline phosphatase (SEAP) using insect cells: Comparison to a batch Sf-9-BEV system

✍ Barbara Ann Jardin; Johnny Montes; Stephane Lanthier; Rosa Tran; Cynthia Elias 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 573 KB

## Abstract The development of insect cells expressing recombinant proteins in a stable continuous manner is an attractive alternative to the BEV system for recombinant protein production. High cell density fed batch and continuous perfusion processes can be designed to maximize the productivity of