A new effective method for the evaluation of glycated intact plasma proteins in diabetic subjects

✍ Scribed by A. Lapolla; D. Fedele; R. Seraglia; S. Catinella; L. Baldo; R. Aronica; P. Traldi

• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 679 KB
• Volume: 38
• Category: Article
• ISSN: 0012-186X
• DOI: 10.1007/bf00402178

✦ Synopsis

The molecular weights of plasma proteins from healthy subjects and from patients with well-or badly-controlled diabetes mellitus have been determined by use of a matrix-assisted laser desorption ionization method, representing a highly accurate technique for the determination of the molecular weight of large biomolecules. Using this approach, different molecular weights of human serum albumin have been found for healthy (66,572-66,694 dalton) and diabetic (66,785-68,959 dalton) subjects. Such differences can be rationalized as being due to the different number of glucose molecules condensed on the protein and/or their further oxida-tion products; in the case of our diabetic patients this number is in the range of 1.4-14.8. The data show the high validity and specificity of the technique, which allows us to evaluate, without any protein degradation procedure, the number of glucose molecules condensed on a specific protein and ascertain the relationship of this number to the physiopathogenetic conditions of the subjects studied.