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A new design of double-barrelled microelectrodes for intracellular pH-measurement in vivo

✍ Scribed by HAGBERG, H. ;LARSSON, S. ;HALJAMÄE, H.

Book ID
114868319
Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
399 KB
Volume
118
Category
Article
ISSN
0001-6772

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✍ K. J. Buckler; R. D. Vaughan-Jones 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 901 KB

We report the use of a new pH-sensitive dualemission fluoroprobe, carboxy-seminaphthorhodafluor-I (carboxy-SNARF-1) for ratiometric recording of intracellular pH (pHi) in small isolated cells. The method is illustrated with pHi measurement in single type-1 cells (cell diameter ~ 10 gm) isolated from