A new culture-free microbiotest for routine detection of cyanobacterial toxins

✍ Scribed by Andrea Kozma Törökné

• Book ID: 101316322
• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 141 KB
• Volume: 14
• Category: Article
• ISSN: 1520-4081
• DOI: 10.1002/(sici)1522-7278(199912)14:5<466::aid-tox3>3.0.co;2-1

✦ Synopsis

The Thamnotoxkit F TM was evaluated for detecting cyanobacterial toxins. This is a 24 hour bioassay using, as test organisms larvae of the freshwater anostracan crustacean Thamnocephalus platyurus hatched from cysts. Freeze-dried Microcystis aeruginosa samples from freshwaters throughout Hungary were tested. Good correlation was obtained between the results of intraperitoneal mouse bioassays and Thamnotoxkit F TM assays. The LC 's for purified microcystins-LR, -RR, and -YR ranged 50 from 0.1 to 2.27 g per mL, respectively in the Thamnotoxkit assay. The LC of laboratory cultures of 50 Cylindrospermopsis raciborskii isolated from samples from Lake Balaton, was 0.574 mg lyophilized material per mL, in a four-laboratory trial. Although not developed originally for detecting cyanobacterial toxins, the Thamnotoxkit F TM has good potential as an initial screening procedure for toxicity assessment of cyanobacterial scums and blooms in freshwaters.