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A New Competitive Fluorescence Assay for the Detection of Patulin Toxin

✍ Scribed by de Champdoré, Marcella; Bazzicalupo, Paolo; De Napoli, Lorenzo; Montesarchio, Daniela; Di Fabio, Giovanni; Cocozza, Immacolata; Parracino, Antonietta; Rossi, Mose'; D'Auria, Sabato

Book ID
120938084
Publisher
American Chemical Society
Year
2007
Tongue
English
Weight
164 KB
Volume
79
Category
Article
ISSN
0003-2700

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A sensitive competitive method was developed for assessing the reactivity of compounds toward glutathione and toward thiols in general. The method employs the reaction of the fluorogenic reagent fluorescein-5-maleimide (FM) with glutathione (GSH) to generate a large increase in fluorescence emission