A New Reporter Gene System Suited for Ce
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RenΓ© Hempel; Frank Wirsching; Andreas Schober; Andreas Schwienhorst
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Article
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2001
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Elsevier Science
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English
β 147 KB
The properties of M-hirudin as a new reporter gene system were examined using rabbit reticulocyte lysate for cell-free protein expression. In contrast to the luciferase gene, in vitro translation of M-hirudin is highly robust against changes in concentrations of K Ψ (and Rb Ψ ). In addition, M-hirud