✦ LIBER ✦
A new benchmark for time-resolved detection of singlet oxygen luminescence – revealing the evolution of lifetime in living cells with low dose illumination
✍ Scribed by J. Schlothauer; S. Hackbarth; B. Röder
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 184 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1612-2011
No coin nor oath required. For personal study only.
✦ Synopsis
The presented setup for singlet oxygen luminescence detection in cell suspensions outperforms the current setups by more than one order-of-magnitude gain in sensitivity. This not only allows precise determination of the singlet oxygen lifetime in cells but also reveals a previously unnoticeable behaviour of the singlet oxygen luminescence during low dose illumination.
Counts, 1000/div. 15 10 5 0 Time, μs Luminescence decay of singlet oxygen photosensitized generated by Jurkat cells incubated with pheophorbide-a