A new assignment of the disulfide linkage in stellacyanin
β Scribed by Helen R. Engeseth; Mark A. Hermodson; David R. McMillin
- Book ID
- 115915673
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 430 KB
- Volume
- 171
- Category
- Article
- ISSN
- 0014-5793
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π SIMILAR VOLUMES
A new procedure is described for identification of disulfide bonds in peptides by fast atom bombardment mass spectrometry (FABMS). Prolonged bombardment of a disulfide-containing peptide in solution by a high-energy xenon beam results in gradual reduction of the disulfide bond. The reduction is the
The thermostable sweet protein brazzein consists of 54 amino acid residues and has four intrumolecular disulfide bonds, the location qf which is unknown. We found that bruzzein resists enzymatic hydrol-vsis at enzyme/substrute ratios ( w / w ) of 1:100-1:10 at 35-40Β°C for 24-48 h. Brazzein wus hjdro