A new assay for fatty acid desaturation
โ Scribed by Barbara R. Talamo; Konrad Bloch
- Publisher
- Elsevier Science
- Year
- 1969
- Tongue
- English
- Weight
- 290 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
A new fluorometric assay method for quinolinic acid is introduced in this study. Quinolinic acid-hydrazine complex, a stable fluorescent compound, is formed after heating quinolinic acid with hydrazine at 215-220ยฐC for 2 min. Fluorescence excitation and emission maxima of the complex are at 285 and
Protein-bound and unbound fatty acids can be efficiently separated at 0 degree C using a hydrophobic column-packing material (Lipidex 1000) similar to the separation of protein-bound and unbound steroids (E. Dahlberg, M. Snochowski, and J.-A. Gustafsson (1980) Anal Biochem. 106, 380-388). Protein-bo