A New Approach to Revealing Point Mutations in DNA Analyzed by Colorimetric Detection
โ Scribed by Marsel Kabilov; Dmitrii Pyshnyi; Grigorii Dymshits; Valentina Zarytova; Evgenia Ivanova
- Publisher
- John Wiley and Sons
- Year
- 2005
- Weight
- 8 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0931-7597
No coin nor oath required. For personal study only.
โฆ Synopsis
Abstract
For Abstract see ChemInform Abstract in Full Text.
๐ SIMILAR VOLUMES
In order to recognize the presence of the R553X point mutation of the cystic fibrosis (CF) gene in the human genome, a peptide nucleic acid (PNA) complementary to the mutated gene tract and bearing three adjacent chiral monomers based on D-lysine (chiral box) was synthesized and used as a probe in C
Several techniques have been developed to screen mismatch repair (MMR) genes for deleterious mutations. Until now, two different techniques were required to screen for both point mutations and large rearrangements. For the first time, we propose a new approach, called "quantitative PCR (qPCR) high-r