N,NΠ-Dicyanomethyl-o-phenylenediamine was synthesized with a 90% yield by a reaction of o-phenylenediamine with chloroacetonitrile in triethylamine. Our experimental results showed that it was the effective fluorogenic substrate for horseradish peroxidase (HRP) and hemin. The K m for the HRP system
A new approach to intramuscular placement of horseradish peroxidase
β Scribed by Kathleen M. Klueber; Dr. Marcia Ontell
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 247 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0148-639X
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4-Phenylylboronic acid enhances the light emission from the horseradish peroxidase catalysed oxidation of luminol by hydrogen peroxide. Optimization studies showed that the greatest enhancement was obtained using micromolar concentrations of the new enhancer. The largest degree of enhancement was fo
The principle that the antigen and the antibody prefer different phases in an aqueous two-phase system is the analytical basis of the work presented here. The antigen horseradish peroxidase, which is bound to a monoclonal antibody (mAb), is separated from free Ag in an aqueous phase system (polyethy