A new approach to determine camptothecin and its analogues affinity to human serum albumin

✍ Scribed by Michał Piotr Marszałł; Adam Buciński; Stefan Kruszewski; Blanka Ziomkowska

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 156 KB
• Volume: 100
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.22318

✦ Synopsis

A novel and fast method for the determination of the binding kinetic data of ligand to protein has been developed. A new tool including human serum albumin-coated magnetic beads (HSA-MB) was used to determine the affinity of camptothecin (CPT) and its analogues to HSA. From the biological activity point of view, these compounds have potential anticancer activity. However, the numerous studies indicate that some of these analogues have a strong affinity to plasma proteins stopping their effective therapy. Thus, the problem of plasma protein binding behavior of CPT's analogues was the subject of this study.