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A new approach to detection of heterozygotes for adenosine deaminase deficiency: A hypothetical method

✍ Scribed by N. Sakura; T. Usui; K. Ito; K. S. Ha; T. Ikeda; H. Yabuuchi; N. Iwanami; A. Komiyama; T. Akabane

Publisher
Springer
Year
1981
Tongue
English
Weight
334 KB
Volume
137
Category
Article
ISSN
0340-6997

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We have substituted deoxyadenosine or adenosine for hypoxanthine in t h e standard HAT selection system in an attempt to select for ADA-normal (ADA+) cells. ADA-human lymphoid line cells could not utilize deoxyadenosine as an alternative to hypoxanthine as a purine source (DAT) and failed to grow bu