A New Approach for in vitro Imaging of Breast Cancer Cells by Anti-Metadherin Targeted PVA-Pyrene
✍ Scribed by E. Ilker Medine; Dilek Odaci; Bahadir N. Gacal; Burcin Gacal; Serhan Sakarya; Perihan Unak; Suna Timur; Yusuf Yagci
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 497 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1616-5187
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✦ Synopsis
Abstract
Poly(vinyl alcohol)‐pyrene‐anti‐metadherin (PVA‐Py‐(Anti‐MTDH)), a novel antibody based water soluble probe containing both fluorescent and target sites in the structure for in vitro imaging of breast cancer cells is reported here. Since breast cancer cells have an excess of MDTH protein expressed on the surface, a PVA‐Py prepared by “Click chemistry” approach is targeted by Anti‐MTDH antibody and applied to the MCF‐7 cell line. After characterization, the designed architecture was evaluated in terms of cell incorporation efficiency and compared with a non‐targeted structure (PVA‐Py). Atomic force microscopy (AFM) and fluorescence microscopy images of cells after incubation of the probe molecules were also obtained to monitor the interaction of the probes with the cancerous cells.
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