𝔖 Bobbio Scriptorium
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A new and rapid triple stain

✍ Scribed by Bonney, Victor

Book ID
104500446
Publisher
Wiley (John Wiley & Sons)
Year
1909
Tongue
English
Weight
118 KB
Volume
13
Category
Article
ISSN
0368-3494

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## Abstract A triple‐stain technique has been developed to score normal acrosome‐reacted human sperm in fixed smears. Live and dead sperm are first differentiated using the vital stain trypan blue. Sperm are then fixed in glutar‐aldehyde, dried onto slides, and the postacrosomal region and acrosome