✦ LIBER ✦

A new absorbed coating for DNA fragment analysis by capillary electrophoresis

✍ Scribed by Marcella Chiari; Marina Cretich; Judit Horvath

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 237 KB
Volume: 21
Category: Article
ISSN: 0173-0835
DOI: 10.1002/(sici)1522-2683(20000501)21:8<1521::aid-elps1521>3.0.co;2-y

No coin nor oath required. For personal study only.

✦ Synopsis

A fully automated coating procedure was devised based on adsorption of a new polymer, poly(dimethylacrylamide-co-allyl glycidyl ether) onto the capillary surface. The whole procedure takes less than 30 min and does not require the use of organic solvents, viscous solutions, or elevated temperature. The coating is stable even under harsh conditions such as alkaline pH, elevated temperature, and denaturant conditions that destroy most other presently available adsorbed coatings. This new adsorbed coating is highly stable and easy to produce in quantity, making it quite unique, and further making it possible to operate with any DNA sieving matrix. Finally, the above-mentioned properties facilitate coating regeneration by a simple wash with a strongly alkaline solution, thus extending the lifetime of the capillary, a highly desirable property for any coating used in biopolymer separations.

📜 SIMILAR VOLUMES

Wall coating for DNA sequencing and frag

Wall coating for DNA sequencing and fragment analysis by capillary electrophoresis

✍ Vladislav Dolník; Diane Xu; Aparna Yadav; John Bashkin; Michele Marsh; Oanh Tu; 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 279 KB 👁 2 views

In capillary electrophoresis, covering the inner capillary surface with a coating is an efficient way to minimize both the electroosmotic flow and sorption of w analytes on the capillary wall. We modified the procedure by Cobb et al. Anal. Ž .x Chem. 62, 2478 1990 for preparing wall coating to perm

Poly-N-hydroxyethylacrylamide (polyDuram

Poly-N-hydroxyethylacrylamide (polyDuramide™): A novel, hydrophilic, self-coating polymer matrix for DNA sequencing by capillary electrophoresis

✍ Methal N. Albarghouthi; Brett A. Buchholz; Piet J. Huiberts; Thomas M. Stein; An 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 272 KB

Performances of new sugar-bearing poly(a

Performances of new sugar-bearing poly(acrylamide) copolymers as DNA sieving matrices and capillary coatings for electrophoresis

✍ Marcella Chiari; Marina Cretich; Sergio Riva; Monica Casali 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 167 KB 👁 2 views

A novel injection method for high-speed

A novel injection method for high-speed proteome analysis by capillary electrophoresis

✍ Mari Tabuchi; Yoshinobu Baba 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 198 KB 👁 1 views

We have developed a new sample injection method for capillary electrophoresis (CE) that reduces the required migration time. We demonstrated a pressurization technique that was performed with buffer in the outlet after the electrokinetic sample injection with no buffer in the outlet. To reduce the m

Spatial open-network formed by mixed tri

Spatial open-network formed by mixed triblock copolymers as a new medium for double-stranded DNA separation by capillary electrophoresis

✍ Tianbo Liu; Dehai Liang; Liguo Song; Vaughn M. Nace; Benjamin Chu 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 199 KB 👁 1 views

Ultra-high throughput rotary capillary a

Ultra-high throughput rotary capillary array electrophoresis scanner for fluorescent DNA sequencing and analysis

✍ James R. Scherer; Indu Kheterpal; Arun Radhakrishnan; William W. Ja; Richard A. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 363 KB 👁 2 views