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A nested polymerase chain reaction for detection of Legionella pneumophila in clinical specimens

✍ Scribed by Sverker Bernander; Hanna-Stina Hanson; Bo Johansson; Lars-Victor von Stedingk


Book ID
111107372
Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
677 KB
Volume
3
Category
Article
ISSN
1198-743X

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Nested polymerase chain reaction for det
✍ Maurizio Zazzi; Laura Romano; Agostina Brasini; Pier Egisto Valensin πŸ“‚ Article πŸ“… 1992 πŸ› John Wiley and Sons 🌐 English βš– 345 KB

## Abstract A highly sensitive two‐step polymerase chain reaction (PCR) method was evaluated for detection of human immunodeficiency virus type 1 (HIV‐1) DNA in clinical specimens. The product resulting from the first amplification reaction is used as the template for the second PCR with an interna