A neosynthesis of sodium channels is involved in the evolution of the sodium current in isolated adult DUM neurons

✍ Scribed by F. Tribut; B. Lapied; A. Duval; M. Pelhate

• Book ID: 104744835
• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 346 KB
• Volume: 419
• Category: Article
• ISSN: 0031-6768
• DOI: 10.1007/bf00370313

✦ Synopsis

Short-term culture of isolated adult dorsal unpaired median (DUM) neurons of the cockroach Periplaneta americana has been used to study the evolution of the sodium current during the time in culture after axotomy and deafferentation treatment. An increase in the maximum peak amplitude of the sodium current recorded under voltage-clump conditions with the patch-clamp technique in the whole-cell recording configuration, was only observed between 24h and 72h (75%) without any modification of the kinetics and the voltage-dependence of the current. A decrease in the level of foetal calf serum in the culture medium reduces the amplitude of the sodium current on all days but does not affect its time-course of development which was on the contrary completely abolished by both protein synthesis inhibitors, actinomycin D and cycloheximide. The results obtained in these neurons strongly suggest that a neosynthesis of sodium cha~nel proteins is involved in the evolution of the sodi~n current induced by axotomy and deafferentation.