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A nanoliter rotary device for polymerase chain reaction

✍ Scribed by Jian Liu; Markus Enzelberger; Stephen Quake

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
183 KB
Volume
23
Category
Article
ISSN
0173-0835

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✦ Synopsis

Polymerase chain reaction (PCR) has revolutionized a variety of assays in biotechnology. The ability to implement PCR in disposable and reliable microfluidic chips will facilitate its use in applications such as rapid medical diagnostics, food control testing, and biological weapons detection. We fabricated a microfluidic chip with integrated heaters and plumbing in which various forms of PCR have been successfully demonstrated. The device uses only 12 nL of sample, one of the smallest sample volumes demonstrated to date. Minimizing the sample volume allows low power consumption, reduced reagent costs, and ultimately more rapid thermal cycling.

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