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A NADH-dependent transketolase assay in erythrocyte hemolysates

โœ Scribed by E.H.J. Smeets; H. Muller; J. De Wael

Book ID
115819192
Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
618 KB
Volume
33
Category
Article
ISSN
0009-8981

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Using a rabbit anti-human transketolase antiserum and Western blotting we can determine nanogram amounts of transketolase in human hemolysates quantitatively. Transketolase concentration in 18 apparently healthy subjects was 55.7 +/- 12.1 micrograms/g Hb (mean +/- SD). Transketolase concentration co