When bovine capillary endothelial (BCE) cells were treated with 10 ng/ml of basic fibroblast growth factor (bFGF) for 10 or 30 minutes at 37"C, washed extensively with phosphate-buffered saline (PBS) and incubated in bFGF-free medium, plasminogen activator (PA) production was stimulated to the same
โฆ LIBER โฆ
A Mutant of Basic Fibroblast Growth Factor that Has Lost the Ability to Stimulate Plasminogen Activator Synthesis in Endothelial Cells
โ Scribed by A. ISACCHI; L. BERGONZONI; M. STATUTO; M. RUSNATI; R. CHIESA; P. CACCIA; P. SARMIENTOS; M. PRESTA; G. RAGNOITI
- Book ID
- 119866718
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 497 KB
- Volume
- 638
- Category
- Article
- ISSN
- 0890-6564
No coin nor oath required. For personal study only.
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Scatchard analysis of binding of 125I-basic fibroblast growth factor (FGF) to baby hamster kidney (BHK) cells revealed the presence of two binding sites: a high affinity site with KD of 20 pM and 80,000 sites per cell and a low affinity site with KD of about 2 nM and 600,000 sites per cell. The bind