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A MultiSite GatewayTMvector set for the functional analysis of genes in the modelSaccharomyces cerevisiae

โœ Scribed by Nagels Durand, Astrid; Moses, Tessa; De Clercq, Rebecca; Goossens, Alain; Pauwels, Laurens


Book ID
119895521
Publisher
BioMed Central
Year
2012
Tongue
English
Weight
248 KB
Volume
13
Category
Article
ISSN
1471-2199

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Generation of null alleles for the funct
โœ Dueรฑas, Encarnacion; Vazquez De Aldana, Carlos R.; De Cos, Teresa; Castro, Carme ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 299 KB ๐Ÿ‘ 3 views

Using PCR-ligated long flanking homology cassettes, null alleles of six open reading frames (ORFs) from chromosome II have been created in Saccharomyces cerevisiae. Deletants were constructed in three genetic backgrounds: FY1679, W303 and CEN.PK2. Tetrad analysis of heterozygous deletants revealed t