๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A multiplex molecular assay for the detection of uniparental disomy for human chromosome 7

โœ Scribed by Emiliano Giardina; Cristina Peconi; Raffaella Cascella; Cecilia Sinibaldi; Valeria Foti Cuzzola; Anna Maria Nardone; Placido Bramanti; Giuseppe Novelli

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
184 KB
Volume
30
Category
Article
ISSN
0173-0835

No coin nor oath required. For personal study only.

โœฆ Synopsis

Abstract

Uniparental disomy (UPD) describes the inheritance of both homologues of a pair of chromosomes from only one parent. This condition is often caused by nondisjunction events during meiosis. UPD has been reported as a rare cause of the autosomal recessive disorder and aberrant expression of imprinted genes that are expressed from only one parental allele, either maternal or paternal. Maternal and/or paternal UPD for chromosome 7 is the most frequently observed UPD after UPD15. Here we developed and validated, for the first time, an effective, CEโ€based method for a rapid and economic detection based on twoโ€fluorescent STR multiplexes.

๐Ÿ“œ SIMILAR VOLUMES

A multiplex molecular assay for the dete
A multiplex molecular assay for the detection of uniparental disomy for human chromosome 15
โœ Emiliano Giardina; Cristina Peconi; Raffaella Cascella; Cecilia Sinibaldi; Anna ๐Ÿ“‚ Article ๐Ÿ“… 2008 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 164 KB

## Abstract Uniparental disomy (UPD) describes the inheritance of both homologues of a pair of chromosomes from only one parent. During the last two decades, the clinical impact of UPD and associated imprinting disorders, such as Praderโ€Willi syndrome (PWS) and Angelman syndrome (AS) increasingly h

A multiplex methylation PCR assay for id
A multiplex methylation PCR assay for identification of uniparental disomy of chromosome 7
โœ Mathew W. Moore; Lisa G. Dietz; Budi Tirtorahardjo; Philip D. Cotter ๐Ÿ“‚ Article ๐Ÿ“… 2003 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 118 KB

Uniparental disomy of chromosome 7 (UPD7) is associated with abnormal phenotypic effects because of inappropriate expression of imprinted genes on chromosome 7. Based on the differential methylation of the promoter region of the imprinted PEG1/MEST locus at 7q32, we designed a multiplex methylation

Development of a chemiluminescent imagin
Development of a chemiluminescent imaging assay for the detection of anti-erythropoietin antibody in human sera
โœ Wenjun Wang; Yanyan Lu; Sichun Zhang; Shidong Wang; Po Cao; Yaping Tian; Xinrong ๐Ÿ“‚ Article ๐Ÿ“… 2009 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 269 KB ๐Ÿ‘ 1 views

## Abstract Measuring low amounts of antiโ€erythropoietin antibodies (antiโ€EPO Abs) is important to evaluate the therapeutic safety of recombinant human erythropoietin (rhEPO). In this work, a simple, sensitive and highโ€throughput chemiluminescent (CL) imaging assay was developed for the detection o

Hybridization Assay at a Disposable Elec
Hybridization Assay at a Disposable Electrochemical Biosensor for the Attomole Detection of Amplified Human Cytomegalovirus DNA
โœ F. Azek; C. Grossiord; M. Joannes; B. Limoges; P. Brossier ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 105 KB

A disposable electrochemical biosensor for the detection of DNA sequences related to the human cytomegalovirus (HCMV) is described. The sensor relies on the adsorption of an amplified human cytomegalovirus DNA strand onto the sensing surface of a screen-printed carbon electrode, and to its hybridiza

A modification of the (75se) selenomethi
A modification of the (75se) selenomethionine assay for the detection of complement-dependent antibody in human tumor systems
โœ Ingalill L. Avis; Frederick P. Avis ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 409 KB

A modification of Brook's prelabeling (7sSE) selenomethionine assay was developed and evaluated for detection of complement-dependent antibody (CDA) in a human tumor system. CDA was indeed detected in some breast cancer patients' sera. To determine whether the assay was reliable and reproducible, xe