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A multiple luminescent procedure for the detection of different papillomaviruses on dot blots

✍ Scribed by Yamina Chikhaoui; Patrick Balaguer; Beatrice Terouanne; Anne-Marie Boussioux; Jean-Claude Nicolas

Book ID
118902505
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
389 KB
Volume
150
Category
Article
ISSN
0022-1759

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A rapid, microanalytical procedure for the reproducible isolation of RNA from small cultured cell samples and application to dot-blot hybridization is described. The procedure employs guanidine hydrochloride solubilization of whole cells, disruption by syringing, and selective precipitation of RNA w