A monoclonal IgMκ from a blood group B individual with specificity for α-galactosyl epitopes on partially hydrolyzed blood group B substance
✍ Scribed by Katherine G. Nickerson; Jerry Liao; Elvin A. Kabat
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 946 KB
- Volume
- 243
- Category
- Article
- ISSN
- 0008-6215
No coin nor oath required. For personal study only.
✦ Synopsis
We have characterized a human monoclonal IgMK, designated IgMDoN, from a blood group B individual. IgM DoN is specific for cY-galactosyl residues on blood group B substance; its fine specificity as defined by hemagglutination, quantitative precipitin, and inhibition ELISA assays was for the defucosylated terminal Gal(al-3)Gal epitope. Gal(crl-3)Gal epitopes are also found on a variety of normal and pathogenic intestinal bacteria, and polyclonal IgG antibodies with the same specificity are found in the serum of nearly all normal individuals. The specificity of IgMDoN was also quite similar to that of a human antiserum, serum 262, obtained by immunizing an individual with blood group B substance that had been subjected to mild acid hydrolysis (BPl). The possible ways whereby IgMDoN might have arisen are discussed.