A monoclonal antibody blocking the Schistosoma mansoni 28-kDa glutathione S-transferase activity reduces female worm fecundity and egg viability
✍ Scribed by Chuan-Bo Xu; Claudie Verwaerde; Jean-Marie Grzych; Josette Fontaine; André Capron
- Book ID
- 102825700
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 788 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0014-2980
No coin nor oath required. For personal study only.
✦ Synopsis
A monoclonal antibody blocking the Schistosoma nulasoni 28-kDa glutathione S-transferase activity reduces female worm fecundity and egg viability"
The protective effects of two different monoclonal antibodies (mAb) raised against the Schistosoma mansoni 28-kDa glutathione S-transferase (Sm 28 GST) were investigated. l h o mAb of the same isotype (IgM) have been selected according to the blocking effect on Sm 28 GSTenzymatic activity (S13) or the lack of blockade (H12).When passively transfered into Fischer rats, both S13 and H12 significantly reduced the worm burden. In BALBlc mice clear effects on female worm fecundity and egg viability were observed when the S13 mAb was transfered; these effects included significantly reduced loads of intestinal eggs, reduced egg hatching rates and an increased proportion of non-living eggs. No effect on egg production and egg hatching was observed in H12-treated mice. In addition, worm pairs recovered from S13-but not H12-treated mice laid significantly fewer eggs in vitro, and normal worm pairs incubated in virro with the S13 mAb produced significantly fewer eggs than those incubated with H12 mAb.
The impairment of egg hatching ability was also reproduced in vitro by the S13 mAb. These data suggest the existence of two different effector mechanisms induced by immunization with Sm 28 GST. The effect on the schistosome worm burden appears to be independent of GST activity whereas the effect on S. mamoni female fecundity and egg viability seems to be significantly linked to the inactivation of the enzymatic site.