A molecular study of dormancy breaking and germination in seeds ofTrollius ledebouri

✍ Scribed by Paul C. Bailey; Grantley W. Lycett; Jeremy A. Roberts

• Book ID: 104615171
• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 685 KB
• Volume: 32
• Category: Article
• ISSN: 0167-4412
• DOI: 10.1007/bf00019110

✦ Synopsis

A cDNA library was generated from seeds of Trollius ledebouri cv. Golden Queen after GA3 treatment. Five clones encoded mRNAs which were down-regulated during dormancy breaking and the initial stages of germination. Two of these showed homology to storage proteins (pPCB3 and pPCB4) and one each to the late-embryogenesisabundant (LEA) group 2 dehydrin proteins (pPCB2), a barley glucose dehydrogenase (pPCB6) and the glutathione S-transferase (GST) superfamily (pPCB7). Transcript levels declined over 8 days in GA3-treated seeds. In dormant imbibed seeds transcript levels were relatively unchanged over the same period except for the PCB3 transcript, the level of which increased.