A Molecular Mechanism of Sickled Erythrocyte Formation
β Scribed by MURAYAMA, MAKIO
- Book ID
- 109635200
- Publisher
- Nature Publishing Group
- Year
- 1964
- Tongue
- English
- Weight
- 434 KB
- Volume
- 202
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/202258a0
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π SIMILAR VOLUMES
Erythrocyte pyruvate kinase (PK) from 5 patients with cogenital non-spherocytic hemolytic and erythrocyte PK deficiency have been studied by immunological methods and electrofucusing. L type immunologically related PK was titrated in crude hemolysate with anti human liver L type PK rabbit serum and
Staining of sickle cells with the fluorescent probes chlortetracycline (a Ca2+ probe) and diindocarbocyanine (a general membrane probe) revealed the presence of Ca2+-containing vesicles which are not found in normal erythrocytes. These vesicles increase in number upon deoxygenation, and are apparent