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A molecular clamp in the crystal structure of the N-terminal domain of the yeast Hsp90 chaperone

✍ Scribed by Prodromou, Chrisostomos; Roe, S. Mark; Piper, Peter W.; Pearl, Laurence H.


Book ID
109964917
Publisher
Nature Publishing Group
Year
1997
Tongue
English
Weight
955 KB
Volume
4
Category
Article
ISSN
1072-8368

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Expression and crystallization of the ye
✍ Prodromou, Chrisostomos; Piper, Peter W.; Pearl, Laurence H. πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 592 KB

Expression of the Saccharomyces cerevisiae Hsp82 chaperone in a pep4-3and hsc82-deficient strain of S. cerevisiae yielded over 25% of the total cell protein as intact Hsp82. Similarly, the amino-terminal domain (residues 1-220) of Hsp82 was expressed to 18% of the total cell protein. Crystals of the