A modified wire-loop method for quantitative electron microscopic radioautography

✍ Scribed by K. Yoshida; F. Murata; S. Ohno; T. Nagata

• Book ID: 104789259
• Publisher: Springer
• Year: 1978
• Tongue: English
• Weight: 624 KB
• Volume: 57
• Category: Article
• ISSN: 1432-119X
• DOI: 10.1007/bf00507359

✦ Synopsis

A simplified method of emulsion coating and development for quantitative electron microscopic radioautography without any special instrument in introduced. Seven grids carrying ultrathin sections are attached to a glass block with double-coated Scotch tape. They are coated with a wire-loop, 2.5 cm in diameter. Ten glass blocks are then stuck on a slide, exposed, developed, fixed and stained simultaneously. Employing this method, the emulsion coating and the following procedures are easily accomplished under an identical condition.